Active Recombinant Human IFNW1 protein, hFc-tagged

• Cat.No.: IFNW1-32H
• Product Overview: Recombinant Human IFNW1 protein(NP_002168.1)(Cys24-Ser195), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is 0.03-0.3 ng/mL.
• Molecular Mass: The recombinant human IFNω/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 409 amino acids with a predicted molecular mass of 46.6 kDa. As a result of glycosylation, rh IFNω/Fc monomer migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Cys24-Ser195
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IFNW1 interferon, omega 1 [ Homo sapiens ]
• Official Symbol: IFNW1
• Synonyms: IFNW1; interferon, omega 1; interferon omega-1; IFN omega 1; interferon omega 1; interferon alpha-II-1; IFN-omega 1, interferon omega-1
• Gene ID: 3467
• mRNA Refseq: NM_002177
• Protein Refseq: NP_002168
• MIM: 147553
• UniProt ID: P05000