Recombinant Human FN1 protein(Ser607-Pro1265), His-tagged

• Cat.No.: FN1-711H
• Product Overview: Recombinant Human FN1(NP_997647.1)(Ser607-Pro1265) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Ser607-Pro1265
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When cells are added to Fibronectin coated plates (2.5 μg/mL and 100 μL/well), approximately 35%-60% cells will adhere specifically after 35 minutes at 37°C.
• Molecular Mass: The secreted recombinant human Fibronectin 1 fragment2 (FN1.2) consists of 670 amino acids and has a calculated molecular mass of 73.2 kDa. As a result of glycosylation, rhFN1.2 migrates as an approximately 94.1 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FN1 fibronectin 1 [ Homo sapiens ]
• Official Symbol: FN1
• Synonyms: FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND; DKFZp686H0342; DKFZp686I1370; DKFZp686F10164; DKFZp686O13149
• Gene ID: 2335
• mRNA Refseq: NM_002026
• Protein Refseq: NP_002017
• MIM: 135600
• UniProt ID: P02751

Reviews

06/21/2021

The preservation conditions are wide and the efficiency is high.

08/14/2019

Experimental process is convenient, which greatly improves the efficiency of scientific research.

03/25/2019

Biological activity of this protein is amazing, and you can see that its production process is very pure, which has a crucial effect on the quality of the product.

Q&As

which organs and tissues is the FN1 protein expressed?

It is expressed in a variety of organs and tissues, including skin, kidneys, cardiovascular system, liver, etc.

How about the FN1 protein in inflammatory diseases?

FN1 protein is involved in the migration, adhesion and activation of inflammatory cells during the inflammatory response, and plays an important role in the development of inflammation.

Have regulators of the FN1 protein been identified?

Yes, multiple factors have been found to regulate the expression and function of FN1 protein, including transcription factors, growth factors, etc.

What is the relationship between FN1 protein and tumor metastasis?

FN1 protein is involved in the invasion and metastasis of tumor cells, which can provide matrix support and guide signals to promote the migration and invasion of tumor cells.

What is the role of FN1 protein in wound healing?

This protein is involved in key steps such as fibrosis, angiogenesis and interstitial cell proliferation in the wound healing process, and plays an important role in wound repair.

How about the mechanism of action of FN1 protein in tissue repair?

The protein plays an important role in key steps such as extracellular matrix remodeling, cell adhesion, and directed differentiation of mesenchymal stem cells during tissue repair.