Recombinant Human TFPI(NP_006278.1)(Met 1-Lys 282) fused with His tag at C-terminal was expressed in HEK293.
Lyophilized from sterile PBS, pH 7.4
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
The secreted recombinant human TFPI comprises 265 amino acids with a predicted molecular mass of 30.6 kDa. As a result of glycosylation, the apparent molecular mass of rhTFPI is approximately 42-45 kDa in SDS-PAGE under reducing conditions.
Met 1-Lys 282
< 1.0 eu per μg of the protein as determined by the LAL method.
Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Store it under sterile conditions at -20 centigrade~-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.