Active Recombinant Human Wilms Tumor 1
Cat.No. : | WT1-1210H |
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Cat. No. : | WT1-1210H. |
Description : | WT-1, the product of Wilms' Tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT-1 protein. Such a complex posttranscriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms' tumor. With the inclusion of exon 5, WT-1 (+KTS) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also colocalizes with splicing factor in a speckled nuclear particle, suggesting that WT-1 protein may function as both a transcription factor and a splicing regulator. The WT-1 protein (residue 1-449, including exon 5 and KTS) is expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography. |
Source : | Insect cells(sf9). |
Form : | Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol. |
Purity : | ≥95% by SDS-PAGE. |
Activity : | 1 ng is the amount sufficient for a gel mobility shift assay in a 20 μl reaction, 50 ng are sufficient for reconstituted transcription assays and 100 ng are sufficient for a protein-protein interaction assay. |
Usage : | For in vitro use only. |
Storage : | Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles. |
Publication : |
The neonatal Fc receptor (FcRn) binds independently to both sites of the IgG homodimer with identical affinity (2015)
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Gene Name : | WT1 Wilms tumor 1 [ Homo sapiens ] |
Synonyms : | WT1; Wilms tumor 1; GUD; AWT1; WAGR; WT33; WIT-2; OTTHUMP00000037553;Wilms tumor protein |
Gene ID : | 7490 |
mRNA Refseq : | NM_000378 |
Protein Refseq : | NP_000369 |
MIM : | 607102 |
UniProt ID : | Q8IYZ5 |
Chromosome Location : | 11p13 |
Function : | RNA binding; double-stranded DNA binding; metal ion binding; promoter binding; protein binding; transcription activator activity; transcription factor activity; zinc ion binding |
Products Types
◆ Recombinant Protein | ||
WT1-1861H | Recombinant Human WT1 Protein (1-449 aa), His-tagged | +Inquiry |
WT1-6261R | Recombinant Rat WT1 Protein, His (Fc)-Avi-tagged | +Inquiry |
WT1-589H | Recombinant Human WT1 Protein, His-tagged | +Inquiry |
WT1-10211M | Recombinant Mouse WT1 Protein, His (Fc)-Avi-tagged | +Inquiry |
WT1-1402H | Recombinant Human WT1 protein, His & GST-tagged | +Inquiry |
◆ Lysates | ||
WT1-278HCL | Recombinant Human WT1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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