Recombinant full length Human Adducin 2 with a N terminal proprietary tag; Predicted MW 105.93 kDa.
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.
726 amino acids
105.930kDa inclusive of tags
Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K562 cells.
pH: 8.00Constituents:0.79% Tris HCl, 0.3% Glutathione
Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
Sequences of amino acids:
MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAAD LRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNN SSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHT ADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLR VSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSS CFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMK WGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGP TCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSAL SSAGGVENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRL GEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEI PATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNAY LRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIE NPNQFVPLYTDPQEVLEMRNKIREQNRQDVKSAGPQSQLL ASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQL TDQELEEYKKEVERKKLELDGEKETAPEEPGSPAKSAPAS PVQSPAKEAETKSPLVSPSKSLEEGTKKTETSKAATTEPE TTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTS KDKTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKK KEKVES
Belongs to the aldolase class II family. Adducin subfamily.