Recombinant Human CLU protein, FLAG-tagged

• Cat.No.: CLU-26745TH
• Product Overview: Recombinant Human CLU fused with FLAG tag at C-terminal was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: FLAG
• Form: Lyophilized. Contains PBS.
• Molecular Mass: 80kDa (under non-reducing conditions); 39kDa and 40kDa (under reducing conditions) (by SDS-PAGE).
• Endotoxin: <0.01EU/μg purified protein (LAL test; Lonza).
• Purity: ≥90% (SDS-PAGE)
• Notes: After opening, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles.
• Stability: Stable for at least 6 months after receipt when stored at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Storage: Short Term Storage: +4 centigrade;
  Long Term Storage: -20 centigrade.
• Concentration: After reconstitution:
  for 10µg size: 0.1mg/ml
  for 50µg size: 1mg/ml
• Reconstitution: 10µg size: Reconstitute with 100µl sterile water.
  50µg size: Reconstitute with 50µl sterile water.
  PBS containing at least 0.1% BSA should be used for further dilutions.

Gene Information

• Gene Name: CLU clusterin [ Homo sapiens ]
• Official Symbol: CLU
• Synonyms: CLU; clusterin; APOJ, CLI, clusterin (complement lysis inhibitor, SP 40,40, sulfated glycoprotein 2, testosterone repressed prostate message 2, apolipoprotein J); apolipoprotein J; complement lysis inhibitor; KUB1; SGP 2; SP 40; sulfated glycoprotein 2; testosterone repressed prostate message 2; TRPM 2; ku70-binding protein 1; aging-associated protein 4; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2; CLI; AAG4; APOJ; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; MGC24903
• Gene ID: 1191
• mRNA Refseq: NM_001831
• Protein Refseq: NP_001822
• MIM: 185430
• UniProt ID: P10909
• Chromosome Location: 8p21-p12
• Pathway: Hemostasis, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Response to elevated platelet cytosolic Ca2+, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem
• Function: NOT ATPase activity; misfolded protein binding; misfolded protein binding; protein binding; ubiquitin protein ligase binding

Reviews

11/30/2021

Impressed with the quality control. This product is a gem in our lab experiments.

01/22/2019

Smooth transactions and swift delivery. Our lab supplies are incomplete without this protein.

04/17/2018

Our lab manager introduced us to this product, and we're never going back. Essential for our studies.

Q&As

How does CLU contribute to the regulation of inflammation, and what role does it play in immune responses and autoimmunity?

CLU has anti-inflammatory properties and modulates immune responses. Its involvement in autoimmunity is linked to its ability to regulate immune cell functions and cytokine production.

How does CLU contribute to the regulation of cell survival and apoptosis, and what are the molecular mechanisms involved?

CLU plays a role in regulating cell survival and apoptosis by interacting with key cellular components, including Bcl-2 family members and caspases, modulating apoptotic pathways.

How does CLU influence cell adhesion and migration, and what role does it play in tissue remodeling and repair processes?

CLU modulates cell adhesion and migration, contributing to tissue remodeling and repair. Its interactions with extracellular matrix components influence cellular behaviors in wound healing and tissue homeostasis.

Can you discuss the association between CLU and neurodegenerative disorders, and how does it impact the pathogenesis of conditions like Alzheimer's disease?

CLU is implicated in neurodegenerative disorders, particularly Alzheimer's disease. Its involvement in processes such as amyloid-beta aggregation and clearance influences disease progression.

Can you elaborate on the molecular interactions of CLU with lipids and its involvement in lipid transport and metabolism?

CLU interacts with lipids, participating in lipid transport and metabolism. Its role in lipoprotein remodeling and cholesterol homeostasis links it to cardiovascular health.