Recombinant Human ITGA4(aa 131 - 275) fused with His tag at N-terminal was expressed in E. coli.
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin 41 and 47 is located on the 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin a4b7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin 47, the gut mucosal homing receptor for peripheral T cells. This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 (IN1) and Integrin 4 (IN2), but not Integrin 4 (CT)).
50mM Tris pH7.5, 150mM NaCl, 5 mM MgCl2
17 kDa (Calculated)
ELISA, WB. This recombinant protein can be used for WB and ELISA.
For research use only.
Store in working aliquots at -70 centigrade. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.