Recombinant Human PDGFRA

• Cat.No.: PDGFRA-1676H
• Product Overview: Recombinant Human PDGFRA encoding the extracellular domain (amino acid residues 1-524) of human PDGF A Type receptor (PDGF Rα) was expressed inNSO cells.

Specification

• Cat. No.: PDGFRA-1676H
• Description: Platelet-derived growth factor receptor, alpha polypeptide, also known as PDGFRA, encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. A soluble form of the platelet-derived growth factor (PDGF) alpha receptor, designated sPDGF-R alpha is capable of binding PDGF ligand and can compete with cell-associated PDGF receptors for ligand binding.
• Molecular Weight: The predicted molecular weight of Recombinant Human PDGF sRαg is Mr 55 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 95-107 kDa.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Purity: >97% by SDS Page and analyzed by silver stain.
• Formulation: 0.2 µm filtered and lyophilized from PBS.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human PDGF sRα was determined by its ability to inhibit the biological activity of hPDGF-AB or rhPDGF-AA in quiescent NR6R-3T3 fibroblasts. The ED50 for this effect is typically 5.0 - 10.0 μg/ml in the presence of 10 ng/ml rhPDGF-AA.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: PDGFRA platelet-derived growth factor receptor, alpha polypeptide [ Homo sapiens ]
• Synonyms: PDGFRA; platelet-derived growth factor receptor, alpha polypeptide; CD140A; PDGFR2; MGC74795; RHEPDGFRA; Alpha-type platelet-derived growth factor receptor; EC 2.7.10.1; PDGF-R-alpha; CD140 antigen-like family member A; CD140a antigen
• Gene ID: 5156
• mRNA Refseq: NM_006206
• Protein Refseq: NP_006197
• MIM: 173490
• UniProt ID: P16234
• Chromosome Location: 4q12
• Pathway: Calcium signaling pathway; Colorectal cancer; Cytokine-cytokine receptor interaction; Endocytosis; Focal adhesion; Gap junction; Glioma; Pathways in cancer; Regulation of actin cytoskeleton; Signaling by PDGF
• Function: ATP binding; nucleotide binding; platelet-derived growth factor alpha-receptor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein heterodimerization activity; receptor activity; transferase activity; vascular endothelial growth factor receptor activity