Recombinant Human THBD protein
Recombinant Human THBD was expressed in HEK293.
Thrombomodulin (TM, CD141, THBD) is an endothelial cell expressed transmembrane glycoprotein that can form a complex with the coagulation factor, Thrombin. The Thrombomodulin/Thrombin complex converts protein C to its activated form, protein Ca, which in turn proteolytically cleaves and deactivates factor Va and factor VIIIa, two essential components of the coagulation mechanism. This inactivation reduces the generation of additional thrombin and thereby effectively prevents continued coagulation. Reduced levels of Thrombomodulin can correlate with the pathogenesis of certain cardiovascular diseases, such as atherosclerosis and thrombosis. However, the serum levels of the truncated circulating form of Thrombomodulin are typically elevated during inflammation and in the presence of various inflammatory related diseases.
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is greater than 500 pmoles/min/ug.
APAEPQPGG SQCVEHDCF ALYPGPATF LNASQICDG LRGHLMTVR SSVAADVIS LLLNGDGGV GRRRLWIGL QLPPGCGDP KRLGPLRGF QWVTGDNNT SYSRWARLD LNGAPLCGP LCVAVSAAE ATVPSEPIW EEQQCEVKA DGFLCEFHF PATCRPLAV EPGAAAAAV SITYGTPFA ARGADFQAL PVGSSAAVA PLGLQLMCT APPGAVQGH WAREAPGAW DCSVENGGC EHACNAIPG APRCQCPAG AALQADGRS CTASATQSC NDLCEHFCV PNPDQPGSY SCMCETGYR LAADQHRCE DVDDCILEP SPCPQRCVN TQGGFECHC YPNYDLVDG ECVEPVDPC FRANCEYQC QPLNQTSYL CVCAEGFAP IPHEPHRCQ MFCNQTACP ADCDPNTQA SCECPEGYI LDDGFICTD IDECENGGF CSGVCHNLP GTFECICGP DSALARHIG TDCDSGKVD GGDSGSGEP PPSPTPGST LTPPA
Endotoxin level is < 0.1ng/ug of protein(<1 EU/ug)
Above 98% as determined by SDS-PAGE Analysis.
Stored at -20 centigrade
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended containing a carrier protein(example 0.1% BSA)and store in working aliquots at -20 centigrade to -80 centigrade.