Recombinant Human TNFSF18(72-199 aa) was expressed in E. coli.
TNFSF18, also known as GITRL, belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It is Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. Recombinant human TNFSF18 protein was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Liquid. In 10mM sodium citrate (pH 3.5), 10% glycerol, 1mMDTT
14.6kDa (129aa), confirmed by MALDI-TOF
MQLETAKEPC MAKFGPLPSK WQMASSEPPC VNKVSDWKLE ILQNGLYLIY GQVAPNANYN DVAPFEVRLY KNKDMIQTLT NKSKIQNVGG TYELHVGDTI DLIFNSEHQV LKNNTYWGII LLANPQFIS
> 90% by SDS - PAGE
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Bradford assay)