Recombinant Human Sphingosine Kinase 1, His-tagged

• Cat.No.: SPHK1-523H
• Product Overview: Recombinant Human Sphingosine kinase 1 (full length) was expressed in aBaculovirus infected Sf9cell expression system with N-terminal His tag. MW=46.9 kDa.

Specification

• Cat. No.: SPHK1-523H
• Description: Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1. Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor and serum.
• Source: Sf9.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: 20 U/μg. Sphingosine kinase activity was determined using Derythro-sphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay buffer: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 μM Naorthovanadate, 0.5 mM ATP, 4 μM Derythro-sphingosine, and 0.1-0.5 μg/ml sphingosine kinase 1.
• Purity: > 80%.
• Stability: > 6 months at –80°C.

Gene Information

• Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
• Synonyms: sphingosine kinase 1; SPHK; SPHK1; EC 2.7.1.91; SPK; SK 1; SPK 1
• Gene ID: 8877
• mRNA Refseq: NM_001142601
• Protein Refseq: NP_001136073
• MIM: 603730
• UniProt ID: Q9NYA1
• Chromosome Location: 17q25.2
• Pathway: Calcium signaling pathway; Fc gamma R-mediated phagocytosis; Metabolic pathways; Sphingolipid metabolism; VEGF signaling pathway
• Function: ATP binding; D-erythro-sphingosine kinase activity; DNA binding; calmodulin binding; diacylglycerol kinase activity; magnesium ion binding; nucleotide binding; sphinganine kinase activity; transferase activity

Reviews

04/16/2023

Its seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.

04/14/2020

With the manufacturer's excellent technical support and its wide range of applications, the SPHK1 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.

01/03/2020

Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

Q&As

How does SPHK1 contribute to drug resistance in cancer?

Elevated SPHK1 levels have been associated with drug resistance in cancer cells, making it a potential target to overcome resistance mechanisms.

How does SPHK1 contribute to immune regulation?

SPHK1 regulates immune cell trafficking and function by modulating S1P levels. This is significant in autoimmune diseases and inflammatory responses.

How might the modulation of SPHK1 impact personalized medicine?

Understanding the role of SPHK1 in different diseases may contribute to the development of personalized therapeutic approaches, tailoring treatments based on individual patient characteristics and disease profiles.

How does SPHK1 influence vascular functions?

SPHK1 is involved in endothelial cell functions and angiogenesis, making it a potential target in the treatment of vascular diseases.

Can SPHK1 inhibitors be used in combination therapies?

Yes, combining SPHK1 inhibitors with other therapeutic agents is being explored to enhance treatment efficacy, especially in cancer.