Recombinant Human YARS, His-tagged

• Cat.No.: YARS-31446TH
• Product Overview: Recombinant fragment, corresponding to amino acids 3-528 of Human Tyrosyl tRNA synthetase with an N-terminal His Tag, approximately 62kDa

Specification

• Description: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:reconstitution with 135 μl distilled water.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium hydrogen phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: DAPSPEEKLHLITRNLQEVLGEEKL KEILKERELKIYWGT ATTGKPHVAYFVPMSKIADFLKAGC EVTILFADLHAYL DNMKAPWELLELRVSYYENVIKAML ESIGVPLEKLKFI KGTDYQLSKEYTLDVYRLSSVVTQH DSKKAGAEVVKQVEH PLLSGLLYPGLQALDEEYLKVDAQF GGIDQRKIFTFAE KYLPALGYSKRVHLMNPMVPGLTGS KMSSSEEESKIDL LDRKEDVKKKLKKAFCEPGNVENNG VLSFIKHVLFPLKSEFVILRDEKWG GNKTYTAYVDLEKDFAAEVVHPGDL KNS VEVALNKLLDPIREKFNTPALKKLA SAAYPDPSKQKPM AKGPAKNSEPEEVIPSRLDIRVGKI ITVEKHPDADSLY VEKIDVGEAEPRTVVSGLVQFVPKE ELQDRLVVVLCNLKP QKMRGVESQGMLLCASIEGINRQVE PLDPPAGSAPGEH VFVKGYEKGQPDEELKPKKKVFEKL QADFKISEECIAQ WKQTNFMTKLGSISCKSLKGGNIS
• Sequence Similarities: Belongs to the class-I aminoacyl-tRNA synthetase family.Contains 1 tRNA-binding domain.

Gene Information

• Gene Name: YARS tyrosyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: YARS
• Synonyms: YARS; tyrosyl-tRNA synthetase; tyrosyl-tRNA synthetase, cytoplasmic; tyrosine tRNA ligase 1; cytoplasmic; tyrRS; YRS; YTS
• Gene ID: 8565
• mRNA Refseq: NM_003680
• Protein Refseq: NP_003671
• MIM: 603623
• Uniprot ID: P54577
• Chromosome Location: 1p35.1
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem
• Function: ATP binding; interleukin-8 receptor binding; ligase activity; nucleotide binding; signal transducer activity