Recombinant Human Growth Differentiation Factor 15

• Cat.No.: GDF15-362H
• Product Overview: Recombinant Human GDF-15 was expressed inE.coliand purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW = 16.7 kDa (151aa).

Specification

• Cat. No.: GDF15-362H
• Description: Growth differentiation factor 15 (GDF-15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. This protein is most abundant in the liver, with lower levels seen in some other tissues. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung.
• Source: E.coli.
• Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMARA RNGDHCPLGP GRCCRLHTVR ASLEDLGWAD WVLSPREVQV TMCIGACPSQ FRAANMHAQI KTSLHRLKPD TVPAPCCVPA SYNPMVLIQK TDTGVSLQTY DDLLAKDCHC I
• Form: Liquid. In 10mM Sodium Citrate (pH3.5) containing 10% glycerol.
• Molecular Weight: 16.7 kDa (151aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
• Purity: > 95% by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Biological activity: Specific activity is 2.8-3.3 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
• Activity Assay: 1. Prepare a 1.mll reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25°C and monitor the A340nm until the value is constant using a spectrophotometer. 3. Add 50ug of recombinant GST into reaction cocktail and mix immediately. 4. Record the increase in A340nm for 5 minutes.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: GDF15 growth differentiation factor 15 [ Homo sapiens ]
• Synonyms: GDF15; growth differentiation factor 15; PDF; MIC1; PLAB; MIC-1;NAG-1; PTGFB; GDF-15; PTGF-beta; prostate differentiation factor; NSAID(nonsteroidal anti-inflammatory drug)-activatedprotein 1; NRG-1; Macrophage inhibitory cytokine 1; NSAID-activated gene1 protein; NSAID-regulated gene 1 protein; Placental TGF-beta; Placentalbone morphogeneticprotein;Growth/differentiation factor 15
• Gene ID: 9518
• mRNA Refseq: NM_004864
• Protein Refseq: NP_004855
• MIM: 605312
• UniProt ID: Q99988
• Chromosome Location: 19p13.11
• Function: cytokine activity; growth factor activity

Reviews

03/19/2021

Dependable protein purification, consistent and cost-effective.

07/29/2019

Precise mass spectrometry analysis, key for proteomics research.

02/22/2019

Great antibody validation, enhances our immunoprecipitation experiments.

Q&As

What role does GDF15 play in cardiovascular diseases, particularly in heart failure and atherosclerosis?

GDF15 influences cardiovascular health, impacting heart failure progression and atherosclerotic processes.

How does GDF15 influence inflammatory processes and immune responses in chronic diseases?

GDF15 modulates inflammatory and immune responses, crucial in the context of chronic diseases.

How does GDF15 contribute to tissue repair and fibrosis in organs like the liver and kidneys?

GDF15 contributes to tissue repair and fibrosis, particularly in liver and kidney pathologies.

What is the impact of GDF15 on cancer development and progression, especially in terms of tumor microenvironment modulation?

GDF15 affects cancer development by altering the tumor microenvironment and influencing tumor cell behavior.

How do alterations in GDF15 expression impact aging and age-related pathologies?

Changes in GDF15 expression are linked to aging and age-related diseases, affecting various physiological processes.

How does GDF15 regulate energy metabolism and appetite in the context of obesity and metabolic disorders?

GDF15 is involved in regulating energy metabolism and appetite, playing a key role in obesity and metabolic syndromes.

What are the mechanisms by which GDF15 affects cellular stress responses and apoptosis?

GDF15 is involved in cellular stress responses, influencing apoptosis and cell survival mechanisms.