Recombinant Human POLR2E

• Cat.No.: POLR2E-521H
• Product Overview: Recombinant human RPB5 is isolated from anE. colistrain that carries the coding sequence of human RPB5 under the control of a T7 promoter.

Specification

• Cat. No.: POLR2E-521H
• Description: hRPB5 (p33) is a highly conserved subunit shared by all three RNA polymerases. It has been shown to be in close contact to promoter DNA when pol II is recruited into the preinitiation complex. RPB5 has also been implicated in direct protein-protein contacts with transcription factor IIB, Rap30 subunit of transcription factor IIF, and gene-specific modulator proteins, such as the hepatitis B virus transactivator protein X or an inhibitor of pol II, RMP (RPB5-mediating protein) .
• Source: E. coli.
• Purity: Purity > 95% bySDS-PAGE analysis.
• Application: RPB5 has been applied in protein-protein interactions assays.
• Endotoxin: 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.
• Buffer: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.

Gene Information

• Gene Name: POLR2E polymerase (RNA) II (DNA directed) polypeptide E, 25kDa [ Homo sapiens ]
• Synonyms: POLR2E; polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; RPB5; XAP4; RPABC1; hRPB25; hsRPB5; POLR2E; DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerases I, II, and III subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; RPB5 homolog
• Gene ID: 5434
• mRNA Refseq: NM_004715
• Protein Refseq: NP_004706
• MIM: 604927
• UniProt ID: Q9Y5B0
• Chromosome Location: 19p13
• Pathway: Huntington"s disease; Metabolic pathways; Purine metabolism; RNA polymerase; Gene Expression; Regulatory RNA pathways; mRNA Processing
• Function: DNA binding; DNA-directed RNA polymerase activity; protein binding; protein kinase activity