Recombinant Human HtrA Serine Peptidase 1, His-tagged

• Cat.No.: HTRA1-2015H
• Product Overview: Recombinant human HtrA1 is expressed in insect cells with a C-terminal His-tag and purified frominsect cellculture supernatants.

Specification

• Cat. No.: HTRA1-2015H
• Description: Secreted human HtrA1 serine protease is supposed to form oligomers of identical protein chains as revealed by X-ray analysis for the bacterial HtrA protein DegP. Polypeptide chains of human HtrA1 consist of several domains: An N-terminal insulin-like growth factor domain is followed by a Kazal-type serine protease inhibitor domain, a linker region, a trypsin-like protease domain and a PDZ domain. The function of HtrA1 appears closely linked to signaling by proteins of the TGFß family [3]. During mouse embryo development HtrA1 is localized in specific areas where signaling by TGFß family proteins occurs. HtrA1 binds TGFß, BMP4, Gdf5 and activin. It inhibits signaling by these factors. For inhibition serine protease activity of HtrA1 is essential. HtrA1 is upregulated in osteoarthritic cartilage. Increased cartilage HtrA1 may possibly aggravate osteoarthritis by antagonizing TGFß and thereby promoting terminal differentiation of articular chondrocytes. In later stages of tumor progression HtrA1 is downregulated.
• Source: insect cells.
• Purity: Recombinant HtrA1 appears as a major protein of about 53 kDa in SDS-PAGE (> 80 % of total protein). Minor bands of HtrA1 fragments may be visible in the enzyme preparation.
• Molecular Form: The calculated Mr of secreted HtrA1 is 50 kDa. HtrA1 is solublized in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 0.05 % Brij-35.
• Enzymatic Activity: Proteolytic activity of recombinant human HtrA1 is documented by digestion of ß-casein. 0.5 mg ß-casein/ml are completely digested by 5 Bg/ml HtrA1 within 3 hours at 37°C.
• Applications: Recombinant HtrA1 allows detailed studies of the structure and function of this protease. The enzyme is used to screen for inhibitors and to characterise inhibitor actions. Recombinant HtrA1 can also serve as standard in enzymatic and immunochemical assays.
• Stability And Storage: Recombinant human HtrA1 is stable until the expiry date given on the label when stored at -70 °C. Repeated freezing and thawing should be avoided.

Gene Information

• Gene Name: HTRA1 HtrA serine peptidase 1 [ Homo sapiens ]
• Synonyms: HtrA serine peptidase 1; L56; HtrA; ARMD7; ORF480; PRSS11; HTRA1; serine protease HTRA1; IGFBP5-protease; protease, serine, 11 (IGF binding); HTRA; Serine protease 11; EC 3.4.21.-
• Gene ID: 5654
• mRNA Refseq: NM_002775
• Protein Refseq: NP_002766
• MIM: 602194
• UniProt ID: Q92743
• Chromosome Location: 10q26.3
• Function: insulin-like growth factor binding; peptidase activity; serine-type endopeptidase activity

Reviews

06/06/2022

The structure of HTRA1 is stable and not easy to mutate, which ensures the reliability of the experimental results.

08/17/2021

The solubility and ion exchange capacity are very good, which is convenient for subsequent experimental processing and purification.

11/10/2020

It is easy to operate and does not require complex operation steps.

Q&As

Is there a relationship between the structure and function of HTRA1 protein?

The structure of the HTRA1 protein determines its function. The serine protease domain is the main functional region of HTRA1 protein, which has catalytic activity and can degrade proteins. In addition, the HTRA1 protein has a number of different domains that are closely related to its function.

Describe the treatment strategy of HTRA1 protein?

Therapeutic strategies for HTRA1 protein include inhibiting its activity, regulating its expression level, and altering its structure. Several drugs are already undergoing clinical trials to evaluate their efficacy against cancer and other diseases.

How can disease be treated by regulating the expression of HTRA1 protein?

There are currently no treatments that directly regulate HTRA1 protein expression. However, some diseases can be treated by inhibiting their activity or regulating their expression levels. For example, some drugs can inhibit the activity of the HTRA1 protein to treat cancer or Alzheimer's disease.

Is HTRA1 involved in cell signal transduction?

HTRA1 protein can be used as a signal transduction molecule by binding to receptors on the cell surface to transmit signals to the cell interior and participate in cell proliferation, differentiation and apoptosis.

How does HTRA1 protein participate in extracellular matrix remodeling?

This protein can degrade a variety of proteins in the extracellular matrix, including collagen, elastin and fibrin, and thus participate in extracellular matrix remodeling.

What method is used to detect the expression level of HTRA1 protein in biological samples?

The expression level of HTRA1 protein in biological samples can be detected by immunohistochemistry, western blot and real-time quantitative PCR.