Active Recombinant Human GSK3B protein, His-tagged

• Cat.No.: GSK3B-616H
• Product Overview: Recombinant Human GSK3B protein(NP_002084.2)(Met1-Thr433), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTAPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.
• Molecular Mass: The recombinant human GSK3B consists of 452 amino acids and predicts a molecular mass of 50.4 kDa. The apparent molecular mass of rhGSK3B is approximately 44-48 kDa in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Thr433
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GSK3B glycogen synthase kinase 3 beta [ Homo sapiens ]
• Official Symbol: GSK3B
• Synonyms: GSK3B; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B
• Gene ID: 2932
• mRNA Refseq: NM_001146156
• Protein Refseq: NP_001139628
• MIM: 605004
• UniProt ID: P49841

Reviews

10/19/2022

In response to the problems in the experiment, the technician gave a satisfactory solution.

05/02/2022

High protein concentrations were received and the desired experimental results were obtained.

04/07/2022

GSK3B proteins purchased are as advertised and the quality of the product is guaranteed.

Q&As

What is the relationship between GSK3B expression and the severity of acute edematous pancreatitis development?

GSK3B expression increased with increasing severity of acute edematous pancreatitis.

What is the relationship between GSK3B and the development of cervical and ovarian cancer?

GSK3B may have the effect of promoting the survival and proliferation of cervical and ovarian cancer tumor cells and inhibiting tumor cell apoptosis.

GSK3B is closely related to the study of which diseases?

GSK3B is closely related to the study of cervical cancer, gastric cancer, breast cancer and other diseases.

What biological processes is GSK3B involved in?

GSK3B is involved in the regulation of cell proliferation, differentiation, survival and apoptosis.

Where is GSK3B located in pancreatic tissue?

In pancreatic tissues, GSK3B is localized to the cytoplasm.