Recombinant Human Neutrophil Cytosolic Factor 4, 40kDa, T7-tagged

• Cat.No.: NCF4-2953H
• Product Overview: Recombinant Human NCF4 was produced inE.coliwith a T7 tag at N-terminus. MW = 39032 Da (1-348 aa).

Specification

• Cat. No.: NCF4-2953H
• Description: Neutrophil cytosol factor 4 is a protein that in humans is encoded by the NCF4 gene. The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Source: E.coli.
• Form: 10 mM Tris. pH 8.0. 0.1% Triton X-100. 0.002% NaN3. 10mM DTT.
• Purity: 95%.
• Applications: MS. SDS.

Gene Information

• Gene Name: NCF4 neutrophil cytosolic factor 4, 40kDa [ Homo sapiens ]
• Synonyms: NCF4; neutrophil cytosolic factor 4, 40kDa; NCF; MGC3810; P40PHOX; SH3PXD4; NCF-4; p40-phox; neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; neutrophil cytosolic factor 4 (40kD)
• Gene ID: 4689
• mRNA Refseq: NM_000631
• Protein Refseq: NP_000622
• MIM: 601488
• UniProt ID: Q15080
• Chromosome Location: 22q13.1
• Pathway: Leishmaniasis; Leukocyte transendothelial migration; Phagosome
• Function: phosphoinositide binding; protein binding; protein dimerization activity

Reviews

01/27/2020

Researchers can confidently incorporate NCF4 protein into their experiments, knowing that they have access to a reliable resource that will meet their experimental needs effectively.

07/11/2018

the NCF4 protein is an excellent choice for researchers due to its high quality, reliability, and the comprehensive technical support provided by the manufacturer.

05/16/2018

The manufacturer of NCF4 protein provides excellent technical support, ensuring that researchers receive prompt assistance and guidance when encountering any challenges or difficulties.

Q&As

How does NCF4 protein interact with other components of the NADPH oxidase complex?

NCF4 protein interacts with other subunits of the NADPH oxidase complex, such as p22phox, p47phox, and p67phox, to assemble the active enzyme complex for ROS production.

What are the implications of NCF4 protein dysfunction in the context of cancer?

Dysregulation of NCF4 protein function may impact the immune response to cancer cells and potentially influence the tumor microenvironment, with potential implications for cancer progression and immunotherapy.

What are the potential therapeutic implications of targeting NCF4 protein?

Targeting NCF4 protein may offer new therapeutic strategies for the treatment of immune disorders and inflammatory conditions by modulating ROS production and immune cell function.

How can NCF4 protein be measured in clinical settings?

NCF4 protein levels can be measured using techniques such as western blotting, ELISA, or flow cytometry in clinical research and diagnostic laboratories.

Can variations in the NCF4 gene affect individual susceptibility to infections?

Genetic variations in the NCF4 gene may influence an individual's susceptibility to infections by impacting the efficiency of phagocyte-mediated killing of pathogens.