Recombinant Human ARL2BP, His-tagged

• Cat.No.: ARL2BP-2878H
• Product Overview: Recombinant human ARL2BP protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography.

Specification

• Cat. No.: ARL2BP-2878H
• Description: ARL2BP is an effector of ADP-ribosylation factor-like 2(ARL2) that is essential for nuclear retention of STAT3. This protein binds to ARL2.GTP with high affinity but does not interact with ARL2.GDP, activated ARF, or RHO proteins. Though predominantly cytosolic, ARL2BP can enter the mitochondria and bind the adenine nucleotide transporter when bound to ARL2. Thus it may also be involved in mitochondria transport and apoptosis.
• Source: E.coli.
• Sequence: MGSSHHHHHH SSGLVPRGSH MDALEGESFA LSFSSASDAE FDAVVGYLED IIMDDEFQLL QRNFMDKYYL EFEDTEENKL IYTPIFNEYISLVEKYIEEQ LLQRIPEFNM AAFTTTLQHH KDEVAGDIFD MLLTFTDFLA FKEMFLDYRA EKEGRGLDLS SGLVVTSLCK SSSLPASQNN LRH.
• Purity: > 90% by SDS – PAGE.
• MW: 32.4 kDa (306aa) confirmed by MALDI-TOF.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 10% Glycerol.
• Concentration: 1mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ARL2BP ADP-ribosylation factor-like 2 binding protein [ Homo sapiens ]
• Synonyms: ARL2BP; ADP-ribosylation factor-like 2 binding protein; BART, BART1; ADP-ribosylation factor-like protein 2-binding protein; ARF-like 2-binding protein; Arf-like 2 binding protein BART1; binder of ARF2 protein 1; binder of Arl Two; binder of Arl2
• Gene ID: 23568
• mRNA Refseq: NM_012106
• Protein Refseq: NP_036238
• UniProt ID: Q9Y2Y0
• Chromosome Location: 16q13
• Function: small GTPase regulator activity

Reviews

05/07/2020

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

01/18/2020

It has demonstrated exceptional effectiveness in WB experiments, showcasing its reliability and precision in detecting and quantifying specific targets.

08/06/2019

With ARL2BP protein, researchers can visualize protein complexes and their interactions with remarkable clarity, unraveling important insights into their functional mechanisms.

01/12/2019

Its exceptional purity and stability make it an ideal choice for a wide range of applications, ensuring reliable and reproducible results.

11/12/2018

Its unique characteristics contribute to obtaining clear and detailed structural information, allowing researchers to gain insights into protein interactions and conformational changes.

11/07/2018

ARL2BP protein has proven to be instrumental in protein electron microscopy structure analysis.

04/06/2018

This protein demonstrates exceptional performance in ELISA, delivering accurate and reliable results in the detection and quantification of specific antigens.

08/24/2017

ARL2BP protein is highly recommended for scientific research applications, especially in ELISA assays and protein electron microscopy structure analysis.

Q&As

Are there any known genetic mutations or variants in the ARL2BP gene?

Yes, genetic variations in the ARL2BP gene have been reported. For example, single nucleotide polymorphisms (SNPs) in the ARL2BP gene have been identified in some population studies. However, the functional consequences of these variants remain to be fully understood.

Is ARL2BP linked to any human diseases?

While the exact role of ARL2BP in human diseases is not well-established, there is emerging evidence suggesting its involvement in certain pathologies. For example, ARL2BP dysregulation has been observed in some cancer types, indicating its potential significance in tumorigenesis.

Does ARL2BP interact with other proteins apart from ARL2?

Yes, ARL2BP has been found to interact with additional proteins. For example, it can bind to BART (beta-amyloid precursor protein (APP)-binding protein), which is involved in the regulation of APP processing and Alzheimer's disease.

Can ARL2BP serve as a therapeutic target?

Currently, there is limited research on targeting ARL2BP for therapeutic purposes. However, further exploration into its functional roles and potential disease associations may reveal its suitability as a therapeutic target in the future.

Can ARL2BP be regulated or modified post-translationally?

Yes, ARL2BP can undergo post-translational modifications, including phosphorylation and ubiquitination. These modifications may regulate its stability, subcellular localization, or interaction with other proteins.

Are there any known functional consequences of ARL2BP deficiency?

ARL2BP deficiency has been associated with certain cellular dysfunctions. Studies have suggested that decreased levels of ARL2BP can lead to impaired mitochondrial fusion and fission dynamics, as well as disruptions in Golgi morphology and protein trafficking.

How is the ARL2BP protein localized within the cell?

ARL2BP is mainly localized to the cytoplasm but can also be found associated with specific cellular membranes, including the Golgi apparatus and the endoplasmic reticulum (ER).