Recombinant Human CTSA protein(Met1-Tyr480), His-tagged

• Cat.No.: CTSA-895H
• Product Overview: Recombinant Human cathepsin A isoform b (Met 1-Tyr 480) (NP_001121167.1) was expressed in HEK293 with a N-terminal signal peptide and a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Tyr480
• Form: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human CTSA existing as a single-chain form consists of 463 amino acids and has a predicted molecular mass of 53 kDa as estimated by SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTSA cathepsin A [ Homo sapiens ]
• Official Symbol: CTSA
• Synonyms: CTSA; cathepsin A; GSL, PPGB, protective protein for beta galactosidase (galactosialidosis); lysosomal protective protein; carboxypeptidase C; carboxypeptidase Y like kininase; carboxypeptidase L; deamidase; lysosomal carboxypeptidase A; urinary kininase; carboxypeptidase-L; beta-galactosidase 2; protective protein cathepsin A; carboxypeptidase Y-like kininase; beta-galactosidase protective protein; GSL; GLB2; NGBE; PPCA; PPGB
• Gene ID: 5476
• mRNA Refseq: NM_000308
• Protein Refseq: NP_000299
• MIM: 613111
• UniProt ID: P10619