Recombinant Human Ras Homolog Gene Family, His-tagged

• Cat.No.: RHOA-3372H
• Product Overview: Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 25.7 kDa(227aa).

Specification

• Cat. No.: RHOA-3372H
• Description: RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila).
• Source: E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT.
• Purity: > 90% by SDS - PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Adherens junction; Axon guidance; Chemokine signaling pathway; Colorectal cancer; Leukocyte transendothelial migration; Neurotrophin signaling pathway; Pathogenic Escherichia coli infection; Regulation of actin cytoskeleton; T cell receptor signaling pathway; TGF-beta signaling pathway; Tight junction; Vascular smooth muscle contraction; Wnt signaling pathway; Signaling by Rho GTPases; Signaling by GPCR; Signalling by NGF
• Publication:
  Utility of Bioluminescent Homogeneous Nucleotide Detection Assays in Measuring Activities of Nucleotide-Sugar Dependent Glycosyltransferases and Studying Their Inhibitors (2021)

Gene Information

• Gene Name: RHOA ras homolog gene family, member A [ Homo sapiens ]
• Synonyms: RHOA; ras homolog gene family, member A; ARHA; ARH12; RHO12; RHOH12; oncogene RHO H12; Aplysia ras-related homolog 12; small GTP binding protein RhoA
• Gene ID: 387
• mRNA Refseq: NM_001664
• Protein Refseq: NP_001655
• MIM: 165390
• UniProt ID: P61586
• Chromosome Location: 3p21.3
• Function: GTP binding; GTPase activity; magnesium ion binding; myosin binding; nucleotide binding; protein binding

Reviews

03/04/2021

Its reliability and versatility make it an essential tool for investigating RHOA protein's expression, function, and structural characteristics.

01/03/2020

The use of RHOA protein in this technique allows researchers to explore the intricate three-dimensional structure of the protein at a molecular level.

12/21/2018

RHOA protein is highly recommended for researchers due to its excellent performance in WB experiments and its utility in protein electron microscopy structure analysis.

Q&As

Is RHOA protein involved in the regulation of blood pressure?

Yes, RHOA is involved in the regulation of vascular smooth muscle contraction and blood pressure.

What are the potential applications of RHOA inhibitors in cancer therapy?

RHOA inhibitors may be used to target the metastatic potential of cancer cells and inhibit their ability to spread to other tissues.

Can RHOA inhibitors be used as potential treatments for cardiovascular diseases?

Yes, RHOA inhibitors may have the potential to be used in the treatment of cardiovascular diseases by targeting vascular smooth muscle contraction.

How is RHOA involved in neurological disorders?

RHOA has been implicated in the pathogenesis of neurological disorders such as Alzheimer's disease and Parkinson's disease.

Are there any clinical trials investigating the use of RHOA inhibitors in neurological disorders?

There is emerging research exploring the potential of RHOA inhibitors as a therapeutic approach for neurological disorders.