Recombinant E.coli rnhA
Cat.No. : | RnhA-739E |
Product Overview : | Recombinant E.coli RNase H (RNaseHI) was over-expressed inE. coliand highly purified. MW is 17.6 kDa. MW = 17.6 kDa. |
- Specification
- Gene Information
- Related Products
Cat. No. : | RnhA-739E |
Description : | Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. |
Source : | E.coli. |
Purity : | Greater than 95% protein determined by SDS-PAGE. |
Form : | 50 units/ul in 20mM Tris-HCl (pH 7.5), 100mM KCl, 1mM DTT, 50% Glycerol. |
Specific Activity : | 100,000 units/mg protein.1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37℃. |
Applications : | 1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA .2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT). 3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA. |
Reagents Supplied With Enzyme : | 100 mM Tris-HCl (pH 8.0), 100 mM MgCl2, 500 mM NaCl, 10 mM DTT, 500 ug/ml BSA (Bovine Serum Albumin). |
Caution : | To avoide contamination of trace amounts of nucleic acids in BSA, use reaction buffer that does not contain BSA and use RNaseH at higher concentrations. |
Storage : | at -20℃. |
Gene Name : | rnhA ribonuclease HI, degrades RNA of DNA-RNA hybrids [ Escherichia coli str. K-12 substr. MG1655 ] |
Synonyms : | rnhA; ribonuclease HI, degrades RNA of DNA-RNA hybrids; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin; EC 3.1.26.4; RNase HI, degrades RNA of DNA-RNA hybrids, participates in DNA replication |
Gene ID : | 946955 |
Protein Refseq : | NP_414750 |
Pathway : | DNA replication |
Function : | DNA-dependent DNA replication; A catabolic process; proteolysis |
Products Types
◆ Recombinant Protein | ||
rnhA-152 | Recombinant E. coli rnhA protein, His-tagged | +Inquiry |
rnhA-1012E | Recombinant E.coli RnhA, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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