Recombinant Human ECHS1, His-tagged

• Cat.No.: ECHS1-2811H
• Product Overview: Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 30.6 kDa (284aa), confirmed by MALDI-TOF.

Specification

• Cat. No.: ECHS1-2811H
• Description: Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid β-oxidation pathway.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ECHS1 enoyl CoA hydratase, short chain, 1, mitochondrial [ Homo sapiens ]
• Synonyms: ECHS1; enoyl CoA hydratase, short chain, 1, mitochondrial; SCEH; mitochondrial short-chain enoyl-coenzyme A hydratase 1; EC 4.2.1.17;OTTHUMP00000020811; Enoyl-CoA hydratase 1; Short chain enoyl-CoA hydratase
• Gene ID: 1892
• mRNA Refseq: NM_004092
• Protein Refseq: NP_004083
• MIM: 602292
• UniProt ID: P30084
• Chromosome Location: 10q26.2-q26.3
• Pathway: Benzoate degradation via CoA ligation; Butanoate metabolism; Caprolactam degradation; Fatty acid elongation in mitochondria; Fatty acid metabolism; Limonene and pinene degradation; Lysine degradation; Propanoate metabolism; Tryptophan metabolism; Valine, leucine and isoleucine degradation ; beta-Alanine metabolism;Lipid and lipoprotein metabolism; Metabolism of amino acids
• Function: enoyl-CoA hydratase activity; enoyl-CoA hydratase activity; lyase activity; protein binding