|Product Overview:||Recombinant Human FLT3LG, Thr27-Pro185, was produced in Sf 9 (baculovirus)-derived in an animal component free process under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
The specific activity of recombinant human Flt-3 Ligand is approximately 1.2 x 10^3 U/μg, which is calibrated against recombinant human Flt-3 Ligand WHO Standard (NIBSC code: 96/532).
|Molecular Mass:||Predicted Molecular Mass: 17.5 kDa
SDS-PAGE: 17-30 kDa, reducing conditions
|N-terminal Sequence Analysis:||Thr-Gln-Asp-(Cys)-Ser-Phe-Gln-His-Ser-Pro|
|Endotoxin:||<0.01 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100-200 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]|
|Synonyms:||FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L;|
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