Human Transferrin

• Cat.No.: TF-4777H
• Product Overview: Product is the lyophilized powder of human TF and buffer salts. Human TF is purified from pooled human serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.

Specification

• Cat. No.: TF-4777H
• Description: TF is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). TF has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe (III) binding sites.
• Source: Human Plasma
• Species: Human
• Preparation: The protein is purified from pooled human plasma and lyophilized from 0.02M NH4HCO3.
• Reconstitution: The protein is readily soluble at 1% in deionised. Alternatively, it can be reconstituted with a neutral buffer, such as PBS.
• SDS-PAGE: The preparation shows a tight cluster of bands with a molecular weight of 77 kDa. Dimers may appear under certan conditions.
• Protein: 98% (By Lowry)
• Purity: > 98 % (By Cellulose Acetate Electrophoresis)
• Iron: 27.85 µg/g (By Atomic Absorption). 1 gm transferrin can bind approximately 1400-1500 µg of iron.
• pH: 7.24 (3% solution)
• Endotoxin Level: 0.034 EU/mg (By Limulus Amebocyte Lysate)
• Microbial detection: No Mycoplasma is detected and Bioburden is less than 10 cfu/ml.
• Moisture: 0.9 % (By Loss on Drying)
• Storage: Store at -10°C or below.
• Pathways: EPHB forward signaling; Formation of Platelet plug; HIF-1-alpha transcription factor network; Hemostasis; Iron uptake and transport; Mineral absorption; Platelet Activation; Platelet degranulation; Response to elevated platelet cytosolic Ca2+; Transferrin endocytosis and recycling; Transmembrane transport of small molecules

Gene Information

• Gene Name: TF transferrin [ Homo sapiens ]
• Official Symbol: TF
• Synonyms: TF; transferring; PRO1557; PRO2086; DKFZp781D0156; serotransferrin; siderophilin; OTTHUMP00000197155; beta-1 metal-binding globulin; Serotransferrin; Beta-1 metal-binding globulin; Siderophilin
• Gene ID: 7018
• mRNA Refseq: NM_001063
• Protein Refseq: NP_001054
• MIM: 190000
• UniProt ID: P02787
• Chromosome Location: 3q21
• Function: ferric iron binding; metal ion binding; protein binding; ubiquitin protein ligase binding