MMP-9 Fluorimetric Assay Kit

• Cat.No.: Kit-3100
• Product Overview: The MMP-9 Assay Kit provides a convenient assay for high throughput screening of MMP-9 inducers and inhibitors. It detects MMP-9 activity in a variety of biological samples using a 5-FAM/QXL520 fluorescence resonance energy transfer (FRET)5 peptide. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon cleavage into two separate fragments by MMP-9 (Scheme 1), the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission wavelengths = 490 nm/520 nm. With excellent fluorescence quantum yield and longer wavelength, 5-FAM shows less interference from autofluorescence of test compounds and cellular components. Compared to an EDANS/DABCYL FRET substrate, this 5-FAM/QXL520 substrate provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Specification

• Storage: Store all kit Components at -20centigrade
  Protect Components A and B from light and moisture
  Components D and E can be stored at 4centigrade for convenience
• Size: 100 Assays