Mouse Anti-Human Acrosomal Vesicle Protein 1 Monoclonal Antibody

• Cat.No.: CAB11477MH
• Product Overview: Mouse monoclonal antibody to human acrosomal vesicle protein 1.

Specification

• Antigen Description: Acrosomal protein SP-10, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. Regulation of cell type-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1 / SP-10 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for< 1% of the total acrosomal vesicle protein 1 message. acrv1 sp-10 is a testis-specific acrosomal protein that has been detected in several species including humans. it may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. acrv1 sp-10 may be involved in sperm-zona binding or penetration. it is also a intra-acrosomal protein that is considered to be a vaccine candidate for>
• Specificity: Human ACRV1. No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA.
• Immunogen: Recombinant Human ACRV1 Protein
• Host: Mouse
• Isotype: Mouse IgG1
• Species: Human
• Clone: 4E3G11
• Purification: Protein A affinity chromatography
• Applications: Western Blot; ELISA
• Dilution: Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human ACRV1 in WB. Using a DAB detection system, the detection limit for Human ACRV1 is approximately 20 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg with the appropriate secondary reagents to detect Human ACRV1. The detection limit for Human ACRV1 is approximately 0.0195 ng/well.
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human ACRV1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: ACRV1 acrosomal vesicle protein 1 [ Homo sapiens ]
• Official Symbol: ACRV1
• Synonyms: ACRV1; acrosomal vesicle protein 1; acrosomal protein SP-10; D11S4365; SP 10; SPACA2; sperm protein 10; SP-10
• Gene ID: 56
• mRNA Refseq: NM_001612
• Protein Refseq: NP_001603
• MIM: 102525
• UniProt ID: P26436
• Chromosome Location: 11p12-q13

Reviews

09/14/2022

The manufacturer's sample program made it easy for me to select the perfect protein for my needs.

10/15/2021

I was relieved to find that the batch control for this protein was impeccable, ensuring that each time I purchase it, the quality is the same.

08/08/2021

I've been a customer of this manufacturer for years and have always been impressed by their consistent high quality.

06/13/2021

The after-sales service was fantastic. The manufacturer was responsive to my inquiries and went above and beyond to ensure my satisfaction.

02/10/2018

The price point was just right for the quality of the protein, and I felt like I was getting excellent value for my money.

Q&As

What is the mechanism of action of the ACRV1 protein?

The mechanism of action of the ACRV1 protein is not well understood, but it may act through the regulation of gene expression or the modulation of signaling pathways.

What is the potential therapeutic significance of targeting the ACRV1 protein?

Targeting the ACRV1 protein may have therapeutic potential in the treatment of autoimmune diseases and other immune-related disorders.

What is the expression pattern of the ACRV1 gene in autoimmune diseases?

The expression of the ACRV1 gene is dysregulated in some autoimmune diseases, such as systemic lupus erythematosus.

How does genetic variation in the ACRV1 gene affect protein function?

The effect of genetic variation on ACRV1 protein function is not well understood, but some SNPs have been associated with an increased risk of autoimmune diseases.

What is the role of the ACRV1 protein in T cell activation?

The ACRV1 protein has been shown to inhibit T cell activation by suppressing the production of cytokines and the proliferation of T cells.

What is the role of the ACRV1 protein in T cell differentiation?

The ACRV1 protein has been shown to promote the differentiation of T cells into regulatory T cells, which are involved in the suppression of immune responses.

What is the genetic variation in the ACRV1 gene?

The ACRV1 gene is polymorphic, with multiple single nucleotide polymorphisms (SNPs) identified in the coding and non-coding regions of the gene.

What is the secondary structure of the ACRV1 protein?

The secondary structure of the ACRV1 protein is predicted to consist of a mixture of alpha-helices and beta-strands.

What is the expression pattern of the ACRV1 gene?

The ACRV1 gene is expressed in a variety of tissues, including the spleen, thymus, and peripheral blood mononuclear cells.

What are the interacting partners of the ACRV1 protein?

The interacting partners of the ACRV1 protein have not been extensively characterized, but the protein may interact with other proteins involved in the regulation of the immune response.