Mouse Anti-Human Cyclin A1 Monoclonal Antibody
Cat.No. : | CAB11491MH |
Product Overview : | Mouse monoclonal antibody to human cyclin A1. |
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Antigen Description : | Cyclin-A1, also known as CCNA1, is a nucleus protein which belongs to the cyclin family and Cyclin AB subfamily. The expression of Cyclin-A1 (CCNA1) is increased in early G1 phase and reaches highest levels during the S and G2/M phases. The mammalian A-type cyclin family consists of two members, Cyclin-A1 (CCNA1) and Cyclin-A2 (CCNA2). CCNA2 promotes both G1/S and G2/M transitions, and targeted deletion of Ccna2 in mouse is embryonic lethal Cyclin-A1 (CCNA1) is expressed in mice exclusively in the germ cell lineage and is expressed in humans at highest levels in the testis and certain myeloid leukaemia cells. Cyclin-A1 (CCNA1) is essential for spermatocyte passage into the first meiotic division in male mice, a function that cannot be complemented by the concurrently expressed B-type cyclins. Cyclin-A1 (CCNA1) may be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. Cyclin-A1 (CCNA1) may function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Cyclin-A1 (CCNA1) interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. |
Specificity : | Human CCNA1 / Cyclin-A1. No cross-reactivity with Baculovirus lysate in ELISA. |
Immunogen : | Recombinant human CCNA1 protein |
Host : | Mouse |
Isotype : | Mouse IgG2a |
Species : | Human |
Clone : | 4G7C3D3 |
Purification : | Protein A affinity chromatography |
Applications : | Western Blot; ELISA |
Dilution : | Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CCNA1 in WB. Using a DAB detection system, the detection limit for human CCNA1 is approximately 8 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human CCNA1. The detection limit for human CCNA1 is 0.0195 ng/well. |
Preparation : | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, insect cell-derived, recombinant human CCNA1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Format : | 0.2 μm filtered solution in PBS with 5% trehalose |
Storage : | This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Gene Name : | CCNA1 cyclin A1 [ Homo sapiens ] |
Official Symbol : | CCNA1 |
Synonyms : | CCNA1; cyclin A1; cyclin-A1; |
Gene ID : | 8900 |
mRNA Refseq : | NM_001111045 |
Protein Refseq : | NP_001104515 |
MIM : | 604036 |
UniProt ID : | P78396 |
Chromosome Location : | 13q12.3-q13 |
Pathway : | APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; |
Function : | protein binding; protein kinase binding; |
Products Types
◆ Recombinant Protein | ||
CCNA1-2996H | Recombinant Human CCNA1 Protein, MYC/DDK-tagged | +Inquiry |
CCNA1-1399M | Recombinant Mouse CCNA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
CCNA1-0647H | Recombinant Human CCNA1 Protein, GST-Tagged | +Inquiry |
Ccna1-795M | Recombinant Mouse Ccna1 Protein, MYC/DDK-tagged | +Inquiry |
CCNA1-642H | Recombinant Human CCNA1 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
CCNA1-682HCL | Recombinant Human CCNA1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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