Native Datura Stramonium Lectin Protein, Fluorescein labeled

Cat.No.: Lectin-1767D
Product Overview: This product is the Fluorescein labeled Datura Stramonium Lectin and has sugar specificity to [GlcNAc]1-3, N-Acetylglucosamine. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Description: DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein. The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0.
Source: Datura stramonium
Species: Datura stramonium
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
Bio-activity: Inhibiting/Eluting Sugar: Chitin Hydrolysate
Molecular Mass: 86 Kda
Applications: Immunofluorescence, Glycobiology
Usage: The recommended concentration range for use is 5-20 µg/ml.
Storage: Refrigerate in the dark. If a precipitate forms upon long-term storage, warm to 37 centigrade. and centrifuge before use.
Concentration: 2mg/ml
Synonyms: Lectin; DSL
UniProt ID: A0A089ZWN7

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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