Native Griffonia Simplicifolia Lectin I Protein, Rhodamine labeled

Cat.No.: Lectin-1729G
Product Overview: This product is the Griffonia (Bandeiraea) Simplicifolia Lectin I and has sugar specificity against Galactose, N-Acetylgalactosamine. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.
Description: GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. This GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
Source: Griffonia Simplicifolia
Species: Griffonia Simplicifolia
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
Bio-activity: Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine.
Carbohydrate-Binding Specificity: Galactose, N-Acetylgalactosamine.
Molecular Mass: 114 kDa
Applications: Immunofluorescence; Glycobiology
Usage: The recommended concentration range for use is 5-20 µg/ml.
Storage: Refrigerate in the dark.
Concentration: 2 mg/ml
Synonyms: Lectin; GSL I; BSL I
UniProt ID: Q8W1R6

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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