Native Narcissus Pseudonarcissus Lectin Protein, Biotinylated

Cat.No.: Lectin-1811N
Product Overview: This product is the Biotinylated Hippeastrum Hybrid (Amaryllis) Lectin and has sugar specificity against Mannose.
Description: NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.
Source: Narcissus Pseudonarcissus
Species: Narcissus Pseudonarcissus
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Bio-activity: Inhibiting Sugar: 500 mM galactose
Molecular Mass: 59 kDa
Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Usage: For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml.
Storage: Refrigerate; for long term storage, aliquot and store frozen.
Concentration: 2mg/ml
Synonyms: Lectin; NPL, NPA
UniProt ID: Q40423

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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