Native Peanut Lectin, FITC conjugated

Cat.No.: Lectin-1719P
Product Overview: This product is arachis hypogaea lectin which against Gal beta 1-3 GalNac (FITC). Affinity purified PNA is conjugated with Fluorescein-5- isothiocyanate (FITC-isomer 1) and purified on Gel filtration column
Description: Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca2+ and Mg2+ per unit.
Inhibiting/Eluting sugars: 200 mM Galactose
Carbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal
Source: Peanut
Species: Peanut
Form: 10 mM bicarbonate, 150 mM NaCl, pH 8.2, 0.1 mM Calcium chloride and 0.05% sodium azide
Applications: ICC/IF.
Immunofluorescence 1:250-1:1000. The optimum dilution should be determined by the individual lab.
Storage: Store at 2-8centigrade.
Concentration: 5 mg/ml (Please refer to the vial label for the specific concentration)
Synonyms: PNA lectin, PNA, Arachis hypogaea, Peanut Lectin, Peanut glutinin ctin, Arachis hypogaea, Peanut agglutinin, Arachis hypogaea lectin, Peanut agglutinin lectin
UniProt ID: P02872

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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