Native Peanut Lectin Protein, Biotinylated

Cat.No.: Lectin-1813P
Product Overview: This product is the Biotinylated Peanut Agglutinin (PNA) Lectin and has sugar specificity against Galactose.
Description: Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.
Source: Peanut
Species: Peanut
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM Ca2+, 0.01 mM Mn2+
Bio-activity: Inhibiting/Eluting Sugar: 200 mM galactose
Molecular Mass: 110 kDa
Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Usage: For most applications we recommend a freshly prepared working solution of 5-20 µg/ml. Use of buffers containing 0.1 mM Ca2+ and 0.01 mM Mn2+ is recommended.
Storage: Refrigerate; for long term storage, aliquots may be stored frozen.
Concentration: 5 mg/ml
Synonyms: Lectin; PNA
UniProt ID: P02872

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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