Native Pisum Sativum Agglutinin Protein, Agarose bound

Cat.No.: Lectin-1827P
Product Overview: This product is the Agarose bound Pisum Sativum Agglutinin (PSA) and has sugar specificity against Mannose and Glucose.
Description: Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity. PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection.
Source: Pisum Sativum
Species: Pisum Sativum
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 0.08% sodium azide, 0.1 M glucose
Bio-activity: Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
Molecular Mass: 53 kDa
Applications: Glycobiology, Affinity Chromatography
Usage: 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Suggested conditions for eluting glycoconjugates: 200 mM α-methyl mannoside/200 mM α-methyl glucoside mixture.
Storage: Refrigerate. DO NOT FREEZE.
Concentration: 3.0 mg/ml of settled gel
Synonyms: Lectin; PSA
UniProt ID: P02867

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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