Active Native Porcine GPT protein

• Cat.No.: GPT-189P
• Product Overview: Native Porcine GPT was purified from Porcine Heart.

Specification

• Description: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
• Source: Porcine Heart
• Species: Porcine
• Form: Lyophilized from tris-HCl, sodium chloride, EDTA, DTE, a-ketoglutarate and phridoxal-5-phosphate
• Bio-activity: > 80 U/mg protein
• Molecular Mass: ~100 kDa
• Unit Definition: One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37 centigrade and pH 7.4.
• Storage: Store at -20 centigrade
• Concentration: > 0.8 mg protein/mg solid (A280)
• Reconstitution: Clear, yellow-green in 10 mg/mL saline.

Gene Information

• Gene Name: GPT glutamic-pyruvate transaminase (alanine aminotransferase) [ Sus scrofa ]
• Official Symbol: GPT
• Synonyms: glutamic-pyruvate transaminase (alanine aminotransferase)
• Gene ID: 100524618
• Chromosome Location: chromosome: 4
• Pathway: 2-Oxocarboxylic acid metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Arginine biosynthesis, organism-specific biosystem