Active Native Vicia Villosa Lectin Protein, Agarose bound
Cat.No. : | Lectin-1855V |
Product Overview : | This product is the Agarose bound Vicia Villosa Lectin and has sugar specificity against N-Acetylgalactosamine. |
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Description : | This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin. |
Source : | Vicia Villosa |
Species : | Vicia Villosa |
Form : | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose 0.08% sodium azide |
Bio-activity : | Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine |
Molecular Mass : | 102 kDa - 144 kDa |
Applications : | Glycobiology, Affinity Chromatography |
Usage : | 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. 2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution. Alternatively, 200 mM N-acetylgalactosamine can be used. Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose. 3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage. |
Storage : | Refrigerate. DO NOT FREEZE. |
Concentration : | 3.0 mg/ml of settled gel |
Synonyms : | Lectin; VVL; VVA |
UniProt ID : | P56625 |
Synonyms : | Lectin; VVL; VVA |
UniProt ID : | P56625 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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