Native Vicia Villosa Lectin Protein, Agarose bound

Cat.No.: Lectin-1855V
Product Overview: This product is the Agarose bound Vicia Villosa Lectin and has sugar specificity against N-Acetylgalactosamine.
Description: This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin.
Source: Vicia Villosa
Species: Vicia Villosa
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose 0.08% sodium azide
Bio-activity: Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
Molecular Mass: 102 kDa - 144 kDa
Applications: Glycobiology, Affinity Chromatography
Usage: 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution. Alternatively, 200 mM N-acetylgalactosamine can be used. Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose.
3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Storage: Refrigerate. DO NOT FREEZE.
Concentration: 3.0 mg/ml of settled gel
Synonyms: Lectin; VVL; VVA
UniProt ID: P56625

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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