Native Wheat Germ Agglutinin Protein, Biotinylated

Cat.No.: Lectin-1860W
Product Overview: This product is the Agarose bound Wheat germ Agglutinin and has sugar specificity against N-Acetylglucosamine.
Description: Wheat germ agglutinin (WGA) contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin is used for the purification of insulin receptors and for neuronal tracing.
Source: Wheat germ
Species: Wheat germ
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+. For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Bio-activity: Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required.
Molecular Mass: 36 kDa
Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Storage: Refrigerate; for long term storage, aliquots may be stored frozen. If a precipitate forms upon long-term storage at 4 centigrade, warm to 37 centigrade. before use.
Concentration: 5 mg/ml
Synonyms: Lectin; WGA

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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