Native Wisteria Floribunda Lectin Protein, Fluorescein labeled

Cat.No.: Lectin-1870W
Product Overview: This product is the Wisteria Floribunda Lectin and and has sugar specificity against N-Acetylgalactosamine. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Description: The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.
Source: Wisteria floribunda
Species: Wisteria floribunda
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM Ca2+
Bio-activity: Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
Molecular Mass: 116 kDa
Applications: Immunofluorescence, Glycobiology
Usage: For most applications we recommend a freshly prepared working solution of 5-20 µg/ml.
Storage: Refrigerate in the dark. If a precipitate forms upon long-term storage at 4 centigrade, warm to 37 centigrade. and centrifuge before use.
Concentration: 2 mg/ml
Synonyms: Lectin; WFA; WFL
UniProt ID: A0A1D5B396

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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