Antigen Description : |
Vascular endothelial growth factor D (VEGF-D), also known as C-fos induced growth factor (FIGF), is a vascular endothelial growth factor. This secreted protein is a member of the platelet derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and performs activities in angiogenesis, lymphangiogenesis, and endothelial cell growth. Human VEGF-D is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. It is structurally and functionally similar to VEGF-C, and is secreted as a non-covelent homodimer in a antiparallel fashion. VEGF-D undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. Like VEGFC, it may particularly be involed in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. |
Specificity : |
Human VEGF-D / FIGF / VEGFD. No cross-reactivity in ELISA with human VEGF-C / Flt4-Land human cell lysate (293 cell line) |
Immunogen : |
Recombinant human VEGF-D protein |
Host : |
Rabbit |
Isotype : |
Rabbit IgG |
Species : |
Human |
Cross Reactivity : |
201 |
Clone : |
A202 |
Applications : |
WB; ELISA |
Dilution : |
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human VEGF-D in WB. Using a DAB detection system, the detection limit for human VEGF-D is approximately 0.5 ng/lane under non-reducing conditions and 50 ng/lane under reducing conditionsELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human VEGF-D. The detection limit for human VEGF-Dis approximately 0.0049 ng/well |
Preparation : |
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human VEGF-D. |
Format : |
0.2 μm filtered solution in PBS with 5% trehalose |
Storage : |
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |