Rabbit Monoclonal Antibody to Human Superoxide Dismutase 1, Soluble

• Cat.No.: CAB11605RH
• Product Overview: Rabbit Monoclonal Antibody to Human Superoxide Dismutase 1, Soluble

Specification

• Antigen Description: Superoxide dismutase [Cu-Zn], also known as superoxide dismutase 1 and SOD1, is an enzyme that in humans is a cytoplasm protein which belongs to the Cu-Zn superoxide dismutase family. SOD1 binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. SOD1 is a soluble cytoplasmic and mitochondrial intermembrane space protein, acting as a homodimer to convert naturally occurring, but harmful, superoxide radicals to molecular oxygen and hydrogen peroxide. SOD1 has a tendency to form fibrillar aggregates in the absence of the intramolecular disulfide bond or of bound zinc ions. These aggregates may have cytotoxic effects. Zinc binding promotes dimerization and stabilizes the native form. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) which is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Death usually occurs within 2 to 5 years. The disease is inherited in 5-10% of cases leading to familial forms.
• Specificity: Human Superoxide Dismutase. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
• Immunogen: Recombinant human SOD1 protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Human
• Clone: A102
• Applications: Western blot; ELISA
• Dilution: Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human SOD1 in WB. Using a DAB detection system, the detection limit for human SOD1 is approximately 0.25 ng/lane under non-reducing conditions and reducing conditionsDirect ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human SOD1. The detection limit for human SOD1 is approximately 0.00245 ng/well
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human SOD1
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SOD1 superoxide dismutase 1, soluble [ Homo sapiens ]
• Official Symbol: SOD1
• Synonyms: SOD1; superoxide dismutase 1, soluble; ALS, ALS1,amyotrophic lateral sclerosis 1 (adult); superoxide dismutase [Cu-Zn]; IPOA; SOD, soluble; indophenoloxidase A; Cu/Zn superoxide dismutase; superoxide dismutase, cystolic; ALS; SOD; ALS1; hSod1; homodimer
• Gene ID: 6647
• mRNA Refseq: NM_000454
• Protein Refseq: NP_000445
• MIM: 147450
• UniProt ID: P00441
• Chromosome Location: 21q22.11
• Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; FOXA1 transcription factor network, organism-specific biosystem; Folate Metabolism, organism-specific biosystem; Hemostasis, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem
• Function: chaperone binding; copper ion binding; metal ion binding; oxidoreductase activity; protein binding; protein homodimerization activity; protein phosphatase 2B binding; superoxide dismutase activity; zinc ion binding

Reviews

11/15/2022

Worked perfectly, no problems.

10/09/2020

Quick delivery, fantastic product.

10/02/2020

Reliable results every time.

Q&As

Are there potential therapeutic strategies targeting SOD1 for disease treatment?

Therapeutic strategies may involve modulating SOD1 activity or expression to mitigate oxidative stress-related damage.

What is the primary biological function of SOD1 in cells?

SOD1 acts as an antioxidant, converting superoxide radicals to hydrogen peroxide and oxygen.

What are the genetic implications of mutations in the SOD1 gene?

Mutations in SOD1 can result in loss or gain of function, contributing to disease development.

How is SOD1 implicated in neurodegenerative diseases?

SOD1 mutations are implicated in Amyotrophic Lateral Sclerosis (ALS), leading to neurodegeneration.

In which cellular compartments is SOD1 primarily located?

SOD1 is primarily located in the cytoplasm and mitochondrial intermembrane space.

What role does SOD1 play in maintaining cellular redox balance?

It helps maintain cellular redox balance, protecting cells from oxidative damage.

How does SOD1 contribute to the cellular antioxidant defense system?

It plays a crucial role in cellular defense against oxidative stress.