Recombinant 2019-nCoV 3CLpro Protein, His-tagged

• Cat.No.: 3CLpro-04C
• Product Overview: Recombinant full-length 2019-nCoV 3CLpro, part of a large replicase polyprotein 1ab (S3264-Q3569), was expressed in E. coli cells with an N-terminal His tag.

Specification

• Description: SARS-CoV-2 3C-like proteinase (3CLpro) is the nonstructural protein number 5, also a cysteine protease, that is released from the virally encoded polyprotein by extensive proteolytic processing. The 3CLpro cleaves the C-terminus of the large replicase polyprotein 1ab (~790 kDa) at no fewer than 11 sites. Inhibiting the activity of this enzyme would block viral replication. The functional importance of 3CLpro in the viral life cycle makes this protease an attractive target for the development of drugs directed against SARS and other coronavirus infections.
• Source: E. coli
• Species: 2019-nCoV
• Tag: His
• Molecular Mass: 35 kDa
• Protein length: 3264-3569
• Purity: > 90%
• Applications: Activity Assay
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 200mM imidazole, 1 mM DTT, 10% glycerol.

Gene Information

• Gene Name: ORF1ab ORF1a polyprotein;ORF1ab polyprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: ORF1ab
• Synonyms: ORF1ab; ORF1a polyprotein;ORF1ab polyprotein; ORF1a polyprotein;ORF1ab polyprotein; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; RNA-dependent RNA polymerase; helicase; 3'-to-5' exonuclease; endoRNAse; 2'-O-ribose methyltransferase; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; nsp11; pp1ab; translated by -1 ribosomal frameshift; pp1a
• Gene ID: 43740578
• Protein Refseq: YP_009725301