Recombinant 3C protease, GST-tagged

• Cat.No.: PSP-138H
• Product Overview: Recombinant HRV14 3C protease was expressed in E.coli with GST tag.

Specification

• Description: 3C Protease is a cysteine protease derived from human rhinovirus. It specifically recognizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein. Recombinant HRV14 3C protease works most effective at 4℃ and can digest substrates at room temperature as well.
• Source: E.coli
• Species: HRV14
• Tag: GST
• Form: Sterile colorless liquid.
• Enzyme Unit: One unit is defined as the amount of enzyme needed to cleave 100μg of fusion protein in 16 hours to 90% completion at 5 centigrade in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.
• Usage: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding Triton X-100, Tween 20 or Nonidet P40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit the activity of protease.
  Cleavage Buffer: 50 mM Tris-HCl, pH 7.0 (at 25 centigrade), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 centigrade prior to use.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening.