Recombinant Bovine Deoxyribonuclease I

• Cat.No.: DNASE1-1227B

Specification

• Cat. No.: DNASE1-1227B
• Description: Deoxyribonuclease I Bovine (bDNase), an enzyme which selectively cleaves DNA. Bovine Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2. Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments.
• Source: Bovine
• Host: Extracted from Pancreas.
• Physical Appearance: Sterile lyophilized freezed dried powder.
• Storage: 2 years when stored at 4°C, three weeks at 15°C, pH-6.3.

Gene Information

• Gene Name: DNASE1 deoxyribonuclease I [ Bos taurus ]
• Official Symbol: DNASE1
• Synonyms: DNASE1; deoxyribonuclease-1; DNase I; NP_776959.1
• Gene ID: 282217
• mRNA Refseq: NM_174534
• Protein Refseq: NP_776959
• UniProt ID: P00639
• Function: actin binding; deoxyribonuclease I activity; endonuclease activity; hydrolase activity

Reviews

12/06/2022

Dependable antibody validation, vital for our immunoprecipitation studies.

01/10/2022

Top-quality protein purification service, essential for our lab's work.

02/22/2019

Fast and reliable protein expression, a critical part of our projects.

Q&As

How does DNASE1 (Deoxyribonuclease I) contribute to DNA degradation and clearance, particularly in apoptotic cells?

DNASE1 helps degrade DNA in apoptotic cells, ensuring proper clearance and preventing inflammation.

How does DNASE1 deficiency lead to the development of systemic lupus erythematosus (SLE) and other autoimmune diseases?

DNASE1 deficiency can contribute to autoimmune diseases like SLE by allowing self-DNA accumulation.

What is the impact of DNASE1 on the regulation of inflammation and immune system activation?

DNASE1 regulates inflammation and immune responses by controlling extracellular DNA levels.

How does DNASE1 influence the resolution of inflammation and tissue repair processes?

DNASE1 aids in resolving inflammation and promoting tissue repair by degrading DNA in damaged tissues.

What are the mechanisms by which DNASE1 modulates the stability and integrity of extracellular DNA?

DNASE1 maintains the stability and integrity of extracellular DNA, preventing inappropriate immune activation.

How do alterations in DNASE1 expression or activity affect susceptibility to chronic inflammatory conditions?

Changes in DNASE1 function can increase susceptibility to chronic inflammation and autoimmune disorders.

What role does DNASE1 play in preventing the accumulation of self-DNA and autoimmune responses?

DNASE1 prevents the accumulation of self-DNA, reducing the risk of autoimmune reactions.