Recombinant Canine CCL8 protein

• Cat.No.: CCL8-29C
• Product Overview: Recombinant Canine CCL8 protein was expressed in E.coli.

Specification

• Description: MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
• Source: E.coli
• Species: Canine
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
• Molecular Mass: Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
• Protein length: 76
• AA Sequence: QPDSVSIPITCCFSMVKRKIPMQKL ESYMRITNSQCPQEAVIFKTKASRE ICADPKQKWVQDYMNHLDQKSQAQK P
• Endotoxin: Less than 0.1 EU/μg of rCaMCP-2/CCL8 as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: CCL8
• Official Symbol: CCL8
• Synonyms: MCP-2
• Gene ID: 448792
• mRNA Refseq: NM_001005255.1
• Protein Refseq: NP_001005255.1
• UniProt ID: Q68AY9