Recombinant Canine VEGFA protein(Met1-Arg190)

• Cat.No.: VEGFA-959C
• Product Overview: Recombinant Canine VEGFA(NP_001103972.1) (Met1-Arg190) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Canine
• Tag: N/A
• Protein length: Met1-Arg190
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized canine VEGF164 / VEGFA at 10 μg/ml (100 μl/well) can bind human VEGFR2-Fc, The EC50 of human VEGFR2-Fc is 33.83-78.95 ng/ml. 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 2-12 ng/mL.
• Molecular Mass: The recombinant canine VEGF164 / VEGFA comprises 164 amino acids and has a predicted molecular mass of 19.1 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Canis lupus familiaris (dog) ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; vascular endothelial growth factor 188
• Gene ID: 403802
• mRNA Refseq: NM_001003175
• Protein Refseq: NP_001003175
• UniProt ID: Q9MYV3

Reviews

04/21/2021

VEGFA's catalytic activity is so strong that it can adapt even in the face of complex chemical reactions.

03/09/2020

VEGFA they produced was highly active and the experimental effect was obvious.

Q&As

How does VEGFA protein relate to other biomarkers?

Studies have shown that there is an association between the expression level of VEGFA protein and other biomarkers, for example, it can be associated with indicators such as tumor cell differentiation, metastatic capacity, and prognosis.

How to harness the activity of VEGFA protein for disease treatment?

At present, a variety of drugs have been developed to target the VEGFA protein and its related signaling pathway, including inhibiting its expression and inhibiting its binding to receptors, etc., for the treatment of tumors and other vascular-related diseases.

What is the role of VEGFA protein in tumor angiogenesis?

VEGFA protein plays a key role in tumor angiogenesis, promoting tumor growth and metastasis by stimulating endothelial cell proliferation, migration, and vascular network formation.

How well is the VEGFA protein expressed in different tissues and organs?

The expression of VEGFA protein in different tissues and organs is different, and it is expressed in tumor tissue, cardiovascular system and other tissues and organs, and the expression level may be affected by many factors.

What is the relationship between the expression level of VEGFA protein and the degree of tumor malignancy?

Studies have shown that the expression level of VEGFA protein is related to the degree of malignancy of the tumor, and the high expression of VEGFA protein often indicates that the tumor is more aggressive.

How about the fuction of VEGFA protein in cardiovascular disease?

VEGFA protein plays an important role in cardiovascular disease by regulating endothelial cell function, promoting vascular repair and maintaining cardiovascular system homeostasis.