Recombinant Chinese hamster GAPDH protein, His-tagged

• Cat.No.: GAPDH-2940C
• Product Overview: Recombinant Chinese hamster GAPDH protein(P17244)(2-333aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Chinese hamster
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 39.7 kDa
• Protein length: 2-333aa
• AA Sequence: VKVGVNGFGRIGRLVTRAAFTSGKV EVVAINDPFIDLNYMVYMFQYDSTH GKFKGTVKAENGKLVINGKAITIFQ ERDPANIKWGDAGAEYVVESTGVFT TMEKAGAHLKGGAKRVIISAPSADA PMFVMGVNQDKYDNSLKIVSNASCT TNCLAPLAKVIHDNFGIVEGLMTTV HAITATQKTVDGPSGKLWRDGRGAA QNIIPASTGAAKAVGKVIPELNGKL TGMAFRVPTPNVSVVDLTCRLEKPA KYEDIKKVVKQASEGPLKGILGYTE DQVVSCDFNSDSHSSTFDAGAGIAL NDNFVKLISWYDNEFGYSNRVVDLM AYMASKE
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.