Recombinant Clostridium Tetani TETX Protein (123-573 aa), His-tagged

• Cat.No.: TETX-937C
• Product Overview: Recombinant Clostridium Tetani (strain Massachusetts/E88) TETX Protein (123-573 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.

Specification

• Description: Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
• Source: E. coli
• Species: Clostridium Tetani
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 55.4 kDa
• Protein length: 123-573 aa
• AA Sequence: SLLDKFDTNSNSVSFNLLEQDPSGA TTKSAMLTNLIIFGPGPVLNKNEVR GIVLRVDNKNYFPCRDGFGSIMQMA FCPEYVPTFDNVIENITSLTIGKSK YFQDPALLLMHELIHVLHGLYGMQV SSHEIIPSKQEIYMQHTYPISAEEL FTFGGQDANLISIDIKNDLYEKTLN DYKAIANKLSQVTSCNDPNIDIDSY KQIYQQKYQFDKDSNGQYIVNEDKF QILYNSIMYGFTEIELGKKFNIKTR LSYFSMNHDPVKIPNLLDDTIYNDT EGFNIESKDLKSEYKGQNMRVNTNA FRNVDGSGLVSKLIGLCKKIIPPTN IRENLYNRTASLTDLGGELCIKIKN EDLTFIAEKNSFSEEPFQDEIVSYN TKNKPLNFNYSLDKIIVDYNLQSKI TLPNDRTTPVTKGIPYAPEYKSNAA STIEIHNIDDNTIYQYLYAQKSPTT L
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.
• UniProt ID: P04958

Gene Information

• UniProt ID: P04958