Recombinant COVID-19 N protein(Met1-Ala419), His-tagged

• Cat.No.: N-1058V
• Product Overview: Recombinant SARS-CoV-2 Nucleocapsid (YP_009724397.2, with mutations P13L, ERS31-33 deletion, R203K, G204R) (Met1-Ala419) was expressed in E. coli with a polyhistidine tag at the N-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.529) which emerged in the South Africa.

Specification

• Source: E. coli
• Species: COVID-19
• Tag: N-His
• Protein length: Met1-Ala419
• Form: Lyophilized from sterile 50mM PB, 500mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant SARS-CoV-2 Nucleocapsid consists of 423 amino acids and predicts a molecular mass of 46.31 kDa.
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.