Active Recombinant Cynomolgus LAG3 protein, Fc-tagged

• Cat.No.: LAG3-1828C
• Product Overview: Recombinant Cynomolgus LAG3 protein(Ala 18 - His 449), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus LAG-3, Fc Tag at 2 μg/mL (100 μL/well) can bind Anti-Human LAG3 MAb, Human IgG1 with a linear range of 8-31 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 73.0 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala18-His449
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.