Recombinant DENV3 E Protein, His-tagged

• Cat.No.: E-05D
• Product Overview: Recombinant Dengue virus serotype 3 E protein incorporating a C-terminal 6×His-tag expressed in mammalian HEK293 mammalian cells, at greater than 95% purity.
  DENV3 Envelope soluble domain, amino acids 279-677 with Gly6 linker and C-terminal His 6-tag, seperated from the protein by a Gly6 linker.

Specification

• Description: Dengue virus is a member of the flavivirus family, which includes Zika virus, West Nile virus and Japanese Encephalitis virus. Many of the proteins expressed by these viruses are very similar, and serological testing for these viruses can be complicated by problems of cross-reactivity. In this context, the use of highly purified antigens, that are glycosylated and folded as native proteins can be highly important in the development of accurate immunoassays.
• Source: HEK293
• Species: DENV3 (Strain: Sri Lanka D3/H/IMTSSA-SRI/2000/1266.)
• Tag: His
• Form: Liquid
• Molecular Mass: 55 kDa
• Protein length: 279-677
• Purity: > 95%
• Storage: Short Term Storage: 2-8 centigrade
  Long Term Storage: -80 centigrade
  Can be frozen, but avoid multiple freeze-thaw cycles.
  Avoid excessive mixing or shocking to prevent aggregation. Long term storage above -80 centigrade may result aggregate formation and/or degradation.
• Concentration: 0.18 mg/mL
• Storage Buffer: 20mM Tris-HCl, 185mM NaCl, pH7.8.
• Shipping: Dry ice