Recombinant E.coli FKBP-type Peptidyl-prolyl Cis-trans Isomerase

• Cat.No.: SlyD-329E
• Product Overview: Recombinant E.Coli slyD was expressed in E.coli and purified by conventional chromatography techniques (196 aa, 21 kDa).

Specification

• Cat. No.: SlyD-329E
• Description: SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
• Source: E.coli.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5).
• Molecular Weight: 21 kDa (196 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
• Biological activity: Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
• Activity Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant SlyD protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: slyD FKBP-type peptidyl-prolyl cis-trans isomerase [ Escherichia coli CFT073 ]
• Synonyms: slyD; FKBP-type peptidyl-prolyl cis-trans isomerase; slyD FKBP-type peptidyl-prolyl cis-trans isomerase.
• Gene ID: 1036353
• Protein Refseq: NP_755987.1
• UniProt ID: P0A9L0

Reviews

11/09/2022

SLYD was found in immunological experiments that it has good antigenicity and can induce target immune response.

10/29/2022

High catalytic efficiency was found in enzyme experiments.

03/04/2022

SLYD is found in biosensor preparation with high sensitivity and specificity.

Q&As

How does SLYD relate to other proteins?

It has a variety of associations with other proteins. For example, it may interact with histone methyltransferases and demethylases to participate in epigenetic regulation, and it may also interact with DNA methyltransferases and DNA demethylases to affect DNA methylation status.

What are the health effects of abnormal expression of SLYD?

Aberrant expression of SLYD may be associated with a variety of diseases, especially the occurrence and progression of tumors. For example, in tumors such as lung, breast, and colon cancers, SLYD expression levels may be abnormally elevated.

How is the level of SLYD detected?

Levels of SLYD can be detected by methods such as immunohistochemistry, western blotting, and real-time PCR, which can assess the amount of SLYD in tissues and cells.

How is SLYD involved in DNA methylation modification?

SLYD affects gene expression by catalyzing DNA methylation modification through the SET domain, which transfers methyl groups to bases of specific DNA sequences.

What conditions might abnormal SLYD levels indicate?

Abnormal SLYD levels may indicate a variety of conditions, particularly neoplastic diseases. For example, in tumors such as lung, breast, and colon cancers, SLYD expression levels may be abnormally elevated.

What is the effect of methylation modification of SLYD on cell function?

Methylation modifications of SLYD can affect the expression of specific genes, which in turn affects processes such as cell growth, differentiation, and apoptosis. For example, in tumor cells, aberrant expression of SLYD may lead to abnormal methylation of genes, which in turn promotes tumor development and progression.